Publications & Presentations

Showing 20 of 500

2026Journal Articles

Optimization and scale up strategies for reproducible AAV enrichment step on CIMmultus® QA HR line

Rok Žigon ; Sara Drmota Prebil ; Tomaž Švigelj ; Kaja Kozjak ; Timotej Žvanut ; Maja Leskovec ; Aleš Štrancar

Gene Therapy

2025Conference Abstracts

Streamlining plasmid linearization for IVT mRNA synthesis with monolith-based immobilized restriction enzyme reactors

Maša Nabergoj ; Špela Peršič Debenjak ; Tina Vodopivec Seravalli ; Polona Megušar ; Urh Černigoj ; Rok Sekirnik ; Manuela Bartolini ; Aleš Štrancar ; Sebastijan Peljhan

PREP, International Symposium on Preparative and Process Chromatography

2025Conference Abstracts

Immobilized enzyme reactors on monoliths

Maša Nabergoj ; Špela Peršič Debenjak ; Tina Vodopivec Seravalli ; Polona Megušar ; Urh Černigoj ; Manuela Bartolini ; Aleš Štrancar ; Sebastijan Peljhan

Biotrans, 17th International Symposium on Biocatalysis and Biotransformations

2025Journal Articles

The impact of chemical reactivity on the quality and stability of RNA–LNP pharmaceuticals

Tristan Kovačič ; Heinrich Haas ; Lior Stotsky-Oterin ; Aleš Štrancar ; Urban Bren ; Dan Peer

Nat. rev., Chem.

2025Journal Articles

Reverse-phase chromatography removes double-stranded RNA, fragments and residual template to decrease immunogenicity and increase cell potency of mRNA and saRNA

Andreja Krušič ; Nina Mencin ; Marta Leban ; Evelin Nett ; Mario Perković ; Ugur Sahin ; Polona Megušar ; Aleš Štrancar ; Rok Sekirnik

Mol. ther., Nucleic acids

2025Journal Articles

Grafting chromatographic monoliths with charged linear polymers for highly productive and selective protein or plasmid DNA purification

Meta Špela Kodermac ; Sara Rotar ; Darko Dolenc ; Klemen Božič ; Aleš Štrancar ; Urh Černigoj

Sep. purif. technol.

2025Journal Articles

Development and validation of AAV capsids separation on specimen columns for reproducibility evaluation of large-scale chromatographic monoliths

Rok Miklavčič ; Tina Simčič ; Sara Rotar ; Polona Komel ; Rok Žigon ; Dona Pavlovič ; Ines Bergoč ; Domen Ipavec ; Ana Simčič Zuljan ; Ažbe Žnidaršič ; Dolores Kukanja ; Jana Vidič ; Aleš Štrancar ; Urh Černigoj

J. sep. sci.

2024Conference Abstracts

Selective hydrophobic interaction chromatography for high purity of supercoiled DNA plasmids

Ajda Sedlar ; Klemen Božič ; Špela Kralj ; Urh Černigoj ; Aleš Štrancar ; Rok Sekirnik

MSS2024

2024Conference Abstracts

Grafting chromatographic monoliths with charged linear polymers for highly productive and selective plasmid DNA purification

Meta Špela Kodermac ; Sara Rotar ; Klemen Božič ; Darko Dolenc ; Aleš Štrancar ; Urh Černigoj

PREP, 36th International Symposium on Preparative and Process Chromatography

2024Conference Abstracts

Evaluation of large-scale monolith chromatographic columns for AAV capsids separation

Rok Miklavčič ; Tina Simčič ; Jure Jakopič ; Sara Rotar ; Rok Žigon ; Darko Dolenc ; Dolores Kukanja ; Jana Vidič ; Maja Leskovec ; Aleš Štrancar ; Urh Černigoj

PREP, 36th International Symposium on Preparative and Process Chromatography

2024Conference Abstracts

Development of immobilized enzyme reactors on monolith backbone for rapid plasmid DNA linearization

Maša Nabergoj ; Špela Peršič Debenjak ; Polona Skrt ; Janja Skok ; Nejc Andlovic ; Urh Černigoj ; Aleš Štrancar ; Manuela Bartolini ; Sebastijan Peljhan

MSS2024

2024Conference Abstracts

Manufacturing of mRNA vaccines

Domen Pregeljc ; Janja Skok ; Tina Vodopivec Seravalli ; Nina Mencin ; Andreja Krušič ; Aleš Štrancar ; Rok Sekirnik

8th Slovenian Pneumology, Allergology and Immunology Congress

2024Journal Articles

Selective hydrophobic interaction chromatography for high purity of supercoiled DNA plasmids

Klemen Božič ; Ajda Sedlar ; Špela Kralj ; Urh Černigoj ; Aleš Štrancar ; Rok Sekirnik

Biotechnol. bioeng.

2024Journal Articles

Determination of linearized pDNA template in mRNA production process using HPLC

Marta Leban ; Tina Vodopivec Seravalli ; Martina Hauer ; Ernst Böhm ; Nina Mencin ; Sandra Potušek ; Andrej Thompson ; Jurij Trontelj ; Aleš Štrancar ; Rok Sekirnik

Anal. bioanal. chem.

2024Journal Articles

Bridging upstream and downstream for improved adenovirus 5 bioprocess

Ivana Petrović Koshmak ; Hana Jug ; Katja Vrabec ; Ana Mavri ; Valentina Novak ; Petra Dekleva ; Veronika Fujs ; Maja Leskovec ; Aleš Štrancar

Electrophoresis

2023Conference Abstracts

Adenovirus vector manufacturing platform using CIMmultus QA column to produce safer products cheaper

Hana Jug ; Katja Vrabec ; Ana Mavri ; Ivana Petrović Koshmak ; Veronika Fujs ; Maja Leskovec ; Aleš Štrancar

15th International Adenovirus Meeting

2023Journal Articles

Production and purification of Newcastle disease virus

Hana Jug ; Natalija Hosta ; Mojca Tajnik ; Aleš Štrancar ; Daniela Brodesser ; Bianca Tisch ; Theresa Heger ; Markus Wolschek ; Joachim Seipelt ; Manfred Reiter

BioProcess int.

2023Journal Articles

Scalable multimodal weak anion exchange chromatographic purification for stable mRNA drug substance

Polona Megušar ; Rok Miklavčič ; Matevž Korenč ; Jure Ličen ; Tina Vodopivec Seravalli ; Urh Černigoj ; Aleš Štrancar ; Rok Sekirnik

Electrophoresis

2023Journal Articles

Preferential exclusion chromatography as a capture step for extracellular AAV harvest from adherent and suspension productions

Maja Leskovec ; Andrej Raspor ; Veronika Fujs ; Andrej Mihevc ; Aleš Štrancar

Electrophoresis

2023Journal Articles

Optimization of rAAV capture step purification using SO3 monolith chromatography

Kaja Bažec ; Mirjam Krašna ; Andrej Mihevc ; Maja Leskovec ; Aleš Štrancar ; Mojca Tajnik

Electrophoresis